ChAT Antibody / Choline acetyltransferase (Antigen affinity purified)

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NSJ Bioreagents
Product Code: NSJ-RQ7061
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7061-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the Choline Acetyltransferase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human U937 cells with Choline Acetyltransferase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Choline Acetyltransferase antibody.~
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Western blot testing of 1) rat brain
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Flow cytometry testing of human U937 cells with Choline Acetyltransferase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Choline Acetyltransferase antibody.~
Western blot testing of 1) rat brain

Documents

Further Information

Application Details:
Western blot: 0.5-1 ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Choline Acetyltransferase antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Choline acetyltransferase (commonly abbreviated as ChAT, but sometimes CAT) is a transferase enzyme responsible for the synthesis of the neurotransmitter acetylcholine. In humans, the choline acetyltransferase enzyme is encoded by the CHAT gene. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids L131-S704) was used as the immunogen for the Choline Acetyltransferase antibody.
Limitation:
This Choline Acetyltransferase antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
P28329