Desmin Antibody [Clone D33] (Purified)

Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Optimal dilution of the Desmin antibody should be determined by the researcher.

Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. IFs are constructed from two-chain α-helical coiled-coil molecules arranged on an imperfect helical lattice, and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. Vimentin is an IF general marker of cells originating in the mesenchyme. Vimentin and Desmin, a related class III IF, are both expressed during skeletal muscle development. Desmin, a 469 amino acid protein found near the Z line in sarcomeres, is expressed more frequently in adult differentiated state tissues. Anti-desmin detects cells of normal smooth, skeletal, and cardiac muscles. Antibody reacts with leiomyomas, leiomyosarcoma, rhabdomyomas, rhabdomyosarcoma, and perivascular cells of glomus tumors of the skin.

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

Human leiomyoma proteins were used as the immunogen for the Desmin antibody.

This Desmin antibody is available for research use only.

Cytoplasmic, membranous

Protein A/G affinity

P17661