Oct4 Antibody / POU5F1 [Clone OCT4/6847R] (Purified)

NSJ Bioreagents
Product Code: NSJ-V4000
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V4000-20UG20 ug£264.00
Quantity:
NSJ-V4000-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG, kappa
Antibody Clonality: Monoclonal
Antibody Clone: OCT4/6847R
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Aliquot the POU5F1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC staining of FFPE human seminoma tissue with OCT4 antibody (clone OCT4/6847R) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.~
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IHC staining of FFPE human seminoma tissue

IHC staining of FFPE human seminoma tissue with OCT4 antibody (clone OCT4/6847R) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.~
IHC staining of FFPE human seminoma tissue

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the POU5F1 antibody should be determined by the researcher.
Description:
OCT4/POU5F1 is a member of the POU family of transcription factors, involved in the regulation of pluripotency during normal development and is detectable in embryonic stem and germ cells. It is a key regulator of self-renewal in embryonic stem cells; its expression is potentially correlated with tumorigenesis and can affect tumor recurrence or resistance to therapies. OCT4/POU5F1 is expressed in undifferentiated pluripotent cells, germ cells in ovary and testis.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant fragment from the human protein was used as the immunogen for the POU5F1 antibody.
Limitation:
This POU5F1 antibody is available for research use only.
Localization:
Nucleus
Purity:
Protein A/G affinity
Uniprot #:
Q01860