GFAP Antibody / Glial Fibrillary Acidic Protein [Clone GFAP/6884] (Purified)

NSJ Bioreagents
Product Code: NSJ-V4004
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V4004-20UG20 ug£264.00
Quantity:
NSJ-V4004-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: GFAP/6884
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the GFAP antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human brain tissue lysate using GFAP antibody (clone GFAP/6884). Predicted molecular weight ~50 kDa.~
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IHC staining of FFPE human cerebellum tissue with GFAP antibody (clone GFAP/6884). HIER: boil tissue sections in

Western blot testing of human brain tissue lysate using GFAP antibody (clone GFAP/6884). Predicted molecular weight ~50 kDa.~
IHC staining of FFPE human cerebellum tissue with GFAP antibody (clone GFAP/6884). HIER: boil tissue sections in

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the GFAP antibody should be determined by the researcher.
Description:
This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A portion of amino acids 150-250 from the human GFAP protein was used as the immunogen for the GFAP antibody.
Limitation:
This GFAP antibody is available for research use only.
Localization:
Cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
P14136