IDH1 Antibody / Isocitrate Dehydrogenase (R132H mutant) [Clone IDH1/7277R] (Purified)

NSJ Bioreagents
Product Code: NSJ-V4026SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V4026SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG, kappa
Antibody Clonality: Monoclonal
Antibody Clone: IDH1/7277R
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Aliquot the Isocitrate Dehydrogenase R132H Mutant antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC staining of FFPE human prostate tissue with IDH1-R132H mutation with Isocitrate Dehydrogenase R132H Mutant antibody (clone IDH1/7277R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human prostate tissue with IDH1-R132H mutation with Isocitrate Dehydrogenase R132H Mutant antibody (clone IDH1/7277R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the Isocitrate Dehydrogenase R132H Mutant antibody should be determined by the researcher.
Description:
IDH1 R132H antibody binds to IDH1-mutated protein, but does not bind the wild-type IDH1 protein. IDH1 R132H point mutations are frequently seen in World Health Organization grade II and III gliomas and are believed to constitute an early step in tumorigenesis.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A recombinant protein fragment specific the the IDH R132H Mutant was used as the immunogen for the Isocitrate Dehydrogenase R132H Mutant antibody.
Limitation:
This Isocitrate Dehydrogenase R132H Mutant antibody is available for research use only.
Localization:
Cytoplasm, nucleus
Purity:
Protein A/G affinity
Uniprot #:
O75874