CUX1 Antibody / Homeobox protein cut-like 1

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NSJ Bioreagents
Product Code: NSJ-RQ7069
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7069-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Homeobox protein cut-like 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with Homeobox protein cut-like 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 6
IHC staining of FFPE human thyroiditis tissue with Homeobox protein cut-like 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 6
IHC staining of FFPE human placental tissue with Homeobox protein cut-like 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 6
IHC staining of FFPE mouse colon tissue with Homeobox protein cut-like 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 6
Western blot testing of 1) human K562, 2) human HeLa, 3) human MCF7, 4) human SH-SY5Y and 5) mouse stomach tissue lysate with Homeobox protein cut-like 1 antibody. Expected molecular weight ~200 kDa with smaller isoforms.
6 / 6
Flow cytometry testing of human HepG2 cells with Homeobox protein cut-like 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Homeobox protein cut-like 1 antibody.
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IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with Homeobox protein cut-like 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human thyroiditis tissue with Homeobox protein cut-like 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human placental tissue with Homeobox protein cut-like 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse colon tissue with Homeobox protein cut-like 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human K562, 2) human HeLa, 3) human MCF7, 4) human SH-SY5Y and 5) mouse stomach tissue lysate with Homeobox protein cut-like 1 antibody. Expected molecular weight ~200 kDa with smaller isoforms.
Flow cytometry testing of human HepG2 cells with Homeobox protein cut-like 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Homeobox protein cut-like 1 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Homeobox protein cut-like 1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
The protein encoded by this gene is a member of the homeodomain family of DNA binding proteins. It may regulate gene expression, morphogenesis, and differentiation and it may also play a role in the cell cycle progession. Several alternatively spliced transcript variants encoding different isoforms have been identified.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-E220) was used as the immunogen for the CUX1 antibody.
Limitation:
This Homeobox protein cut-like 1 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic (Golgi)
Purity:
Antigen affinity purified
Uniprot #:
P39880