JAM-A Antibody / Junctional adhesion molecule 1

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NSJ Bioreagents
Product Code: NSJ-RQ7077
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7077-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Junctional adhesion molecule A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 5
IHC staining of FFPE human colonic adenocarcinoma tissue with Junctional adhesion molecule A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human tonsil tissue with Junctional adhesion molecule A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
Immunofluorescent staining of FFPE human T-47D cells with Junctional adhesion molecule A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
4 / 5
Western blot testing of human 1) HaCaT, 2) T-47D, 3) A431 and 4) RT4 cell lysate with Junctional adhesion molecule A antibody. Expected molecular weight: 35~43 kDa depending on glycosylation level.
5 / 5
Flow cytometry testing of human Daudi cells with Junctional adhesion molecule A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Junctional adhesion molecule A antibody.
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IHC staining of FFPE human colonic adenocarcinoma tissue with Junctional adhesion molecule A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with Junctional adhesion molecule A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human T-47D cells with Junctional adhesion molecule A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HaCaT, 2) T-47D, 3) A431 and 4) RT4 cell lysate with Junctional adhesion molecule A antibody. Expected molecular weight: 35~43 kDa depending on glycosylation level.
Flow cytometry testing of human Daudi cells with Junctional adhesion molecule A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Junctional adhesion molecule A antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Junctional adhesion molecule A antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Junctional adhesion molecule A is a protein that in humans is encoded by the F11R gene. Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor. Multiple 5' alternatively spliced variants, encoding the same protein, have been identified but their biological validity has not been established.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids S28-T294) was used as the immunogen for the Junctional adhesion molecule A antibody.
Limitation:
This Junctional adhesion molecule A antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
Q9Y624