Ataxin 1 Antibody / ATXN1

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NSJ Bioreagents
Product Code: NSJ-RQ7132
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7132-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 2B13G8
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Ataxin 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with Ataxin 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 9
IHC staining of FFPE human thyroiditis tissue with Ataxin 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 9
IHC staining of FFPE human hepatocellular carcinoma tissue with Ataxin 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE mouse brain tissue with Ataxin 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 9
IHC staining of FFPE rat brain tissue with Ataxin 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 9
Western blot testing of 1) human HepG2, 2) human 293T, 3) human A431, 4) human PC-3, 5) rat brain, 6) rat liver, 7) mouse brain and 8) mouse liver tissue lysate with Ataxin 1 antibody. Commonly observed molecular weight: 87-105 kDa.
7 / 9
Flow cytometry testing of human PC-3 cells with Ataxin 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ataxin 1 antibody.
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Flow cytometry testing of mouse ANA-1 cells with Ataxin 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ataxin 1 antibody.
9 / 9
Flow cytometry testing of rat NRK cells with Ataxin 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ataxin 1 antibody.
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IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with Ataxin 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human thyroiditis tissue with Ataxin 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human hepatocellular carcinoma tissue with Ataxin 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with Ataxin 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with Ataxin 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HepG2, 2) human 293T, 3) human A431, 4) human PC-3, 5) rat brain, 6) rat liver, 7) mouse brain and 8) mouse liver tissue lysate with Ataxin 1 antibody. Commonly observed molecular weight: 87-105 kDa.
Flow cytometry testing of human PC-3 cells with Ataxin 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ataxin 1 antibody.
Flow cytometry testing of mouse ANA-1 cells with Ataxin 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ataxin 1 antibody.
Flow cytometry testing of rat NRK cells with Ataxin 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ataxin 1 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the Ataxin 1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Ataxin-1 is a protein that in humans is encoded by the ATXN1 gene. It is a causative factor for spinocerebellar ataxia type 1 (SCA1), and the related Brother of ATXN1 (BOAT1), are human proteins involved in transcriptional repression. ATXN1 and BOAT1 might participate in several Notch-controlled developmental and pathological processes.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
C-terminal amino acids ESRKLEKSEDEPPLTLPKPSLIPQEVKICIE were used as the immunogen for the Ataxin 1 antibody.
Limitation:
This Ataxin 1 antibody is available for research use only.
Localization:
Cytoplasm, Nucleus
Purity:
Antigen affinity purified
Uniprot #:
P54253