WDR1 Antibody / WD repeat-containing protein 1

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NSJ Bioreagents
Product Code: NSJ-RQ7133
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7133-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 5C11C8
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the WD repeat-containing protein 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human colorectal adenocarcinoma tissue with WD repeat-containing protein 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 3
IHC staining of FFPE human liver cancer tissue with WD repeat-containing protein 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 3
Western blot testing of 1) human U-87 MG, 2) human A549, 3) mouse lung and 4) mouse liver tissue lysate with WD repeat-containing protein 1 antibody. Expected molecular weight ~66 kDa.
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IHC staining of FFPE human colorectal adenocarcinoma tissue with WD repeat-containing protein 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with WD repeat-containing protein 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human U-87 MG, 2) human A549, 3) mouse lung and 4) mouse liver tissue lysate with WD repeat-containing protein 1 antibody. Expected molecular weight ~66 kDa.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml
Application Note:
Optimal dilution of the WD repeat-containing protein 1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
WD repeat-containing protein 1 is a protein that in humans is encoded by the WDR1 gene. It is mapped to 4p16.1. This gene encodes a protein containing 9 WD repeats. WD repeats are approximately 30- to 40-amino acid domains containing several conserved residues, mostly including a trp-asp at the C-terminal end. WD domains are involved in protein-protein interactions. The encoded protein may help induce the disassembly of actin filaments. Two transcript variants encoding different isoforms have been found for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
C-terminal amino acids MDMMVYVWTLSDPETRVKIQDAHRLHHV were used as the immunogen for the WD repeat-containing protein 1 antibody.
Limitation:
This WD repeat-containing protein 1 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
O75083