Adam17 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ7140
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7140-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
After reconstitution, the Adam17 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Western blot testing of 1) rat heart, 2) rat skeletal muscle, 3) rat thymus, 4) rat C6, 5) mouse heart, 6) mouse skeletal muscle, 7) mouse thymus and 8) mouse C2C12 cell lysate with Adam17 antibody. Expected molecular weight: 74-130 kDa depending on level of glycosylation
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Western blot testing of 1) rat heart, 2) rat skeletal muscle, 3) rat thymus, 4) rat C6, 5) mouse heart, 6) mouse skeletal muscle, 7) mouse thymus and 8) mouse C2C12 cell lysate with Adam17 antibody. Expected molecular weight: 74-130 kDa depending on level of glycosylation

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Adam17 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
ADAM17 (ADAM metallopeptidase domain 17), also called TACE (tumor necrosis factor-alpha-converting enzyme), is a 70-kDa enzyme that belongs to the ADAM protein family of disintegrins and metalloproteases. Expression studies showed that the encoded protein cleaves precursor tumor necrosis factor-alpha to its mature form. Northern blot analysis revealed that the gene was expressed as a 5-kb mRNA in all tissues examined. ADAM17 is understood to be involved in the processing of tumor necrosis factor alpha (TNF-a) at the surface of the cell, and from within the intracellular membranes of the trans-Golgi network. This process, which is also known as 'shedding', involves the cleavage and release of a soluble ectodomain from membrane-bound pro-proteins (such as pro-TNF-a), and is of known physiological importance. ADAM17 was the first 'sheddase' to be identified, and is also understood to play a role in the release of a diverse variety of membrane-anchored cytokines, cell adhesion molecules, receptors, ligands, and enzymes.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant mouse protein (amino acids R215-M449) was used as the immunogen for the Adam17 antibody.
Limitation:
This Adam17 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
Q9Z0F8