Alpha Fetoprotein / AFP Antibody

NSJ Bioreagents
Product Code: NSJ-RQ7141
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7141-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
After reconstitution, the Alpha Fetoprotein antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Western blot testing of human 1) HepG2 and 2) HCCP cell lysate with Alpha Fetoprotein antibody. Predicted molecular weight: ~70 kDa.

Western blot testing of human 1) HepG2 and 2) HCCP cell lysate with Alpha Fetoprotein antibody. Predicted molecular weight: ~70 kDa.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Alpha Fetoprotein antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
AFP, also called Alpha-fetoprotein, is a protein that in humans is encoded by the AFP gene. It is mapped to 4q13.3. The level of AFP in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. In rodents AFP binds estradiol to prevent the transport of this hormone across the placenta to the fetus. The main function of this is to prevent the virilization of female fetuses. Moreover, it has an important role as a diagnostic marker, especially in certain tumors and liver diseases of childhood. AFP is also used to test the potential usefulness of plasma alpha fetoprotein determination as a diagnostic marker in biliary atresia, hepatitis, and yolk sac derived tumours.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids R19-L258) was used as the immunogen for the Alpha Fetoprotein antibody.
Limitation:
This Alpha Fetoprotein antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
P02771