ALYREF Antibody / Tho Complex 4

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NSJ Bioreagents
Product Code: NSJ-RQ7142
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7142-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the ALYREF antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 3
Immunofluorescent staining of FFPE human A431 cells with ALYREF antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human placenta, 2) human PC-3, 3) human HeLa, 4) human HepG2, 5) human 293T, 6) human A431, 7) human A549, 8) rat brain, 9) rat liver, 10) rat PC-12, 11) rat NRK, 12) mouse brain, 13) mouse liver, 14) mouse Neuro-2a and 15) mouse RAW264.7 cell lysate with ALYREF antibody. Predicted molecular weight ~27 kDa.
3 / 3
Flow cytometry testing of human SiHa cells with ALYREF antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ALYREF antibody.
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Immunofluorescent staining of FFPE human A431 cells with ALYREF antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human placenta, 2) human PC-3, 3) human HeLa, 4) human HepG2, 5) human 293T, 6) human A431, 7) human A549, 8) rat brain, 9) rat liver, 10) rat PC-12, 11) rat NRK, 12) mouse brain, 13) mouse liver, 14) mouse Neuro-2a and 15) mouse RAW264.7 cell lysate with ALYREF antibody. Predicted molecular weight ~27 kDa.
Flow cytometry testing of human SiHa cells with ALYREF antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ALYREF antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the ALYREF antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Aly/REF export factor, also known as THO complex subunit 4, THOC4 and Tho4, is a protein that in humans is encoded by the ALYREF gene. The protein encoded by this gene is a heat stable, nuclear protein and functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-S257) was used as the immunogen for the ALYREF antibody.
Limitation:
This ALYREF antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q86V81