AMOT Antibody / Angiomotin

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NSJ Bioreagents
Product Code: NSJ-RQ7143
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7143-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the Angiomotin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human Caco-2 cells with Angiomotin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Western blot testing of human 293T cell lysate with Angiomotin antibody. Predicted molecular weight: 118-130 kDa (p130 isoform) and 72-80 kDa (p80 isoform).
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Flow cytometry testing of human Caco-2 cells with Angiomotin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Angiomotin antibody.
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Immunofluorescent staining of FFPE human Caco-2 cells with Angiomotin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 293T cell lysate with Angiomotin antibody. Predicted molecular weight: 118-130 kDa (p130 isoform) and 72-80 kDa (p80 isoform).
Flow cytometry testing of human Caco-2 cells with Angiomotin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Angiomotin antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Angiomotin antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Angiomotin (AMOT) is a protein that in humans is encoded by the AMOT gene. This gene belongs to the motin family of angiostatin binding proteins characterized by conserved coiled-coil domains and C-terminal PDZ binding motifs. The encoded protein is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells toward growth factors during the formation of new blood vessels. Alternative splicing results in multiple transcript variants encoding different isoforms.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids T12-Q622) was used as the immunogen for the Angiomotin antibody.
Limitation:
This Angiomotin antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
Q4VCS5