ARHGAP45 Antibody / Rho GTPase-activating protein 45

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NSJ Bioreagents
Product Code: NSJ-RQ7145
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7145-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the ARHGAP45 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human B lymphocytic tumor tissue with ARHGAP45 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 3
Western blot testing of human 1) HeLa, 2) Raji and 3) A549 cell lysate with ARHGAP45 antibody. Predicted molecular weight ~125 kDa but can be observed at higher molecular weights.
3 / 3
Flow cytometry testing of human SiHa cells with ARHGAP45 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ARHGAP45 antibody.
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IHC staining of FFPE human B lymphocytic tumor tissue with ARHGAP45 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HeLa, 2) Raji and 3) A549 cell lysate with ARHGAP45 antibody. Predicted molecular weight ~125 kDa but can be observed at higher molecular weights.
Flow cytometry testing of human SiHa cells with ARHGAP45 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ARHGAP45 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the ARHGAP45 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Predicted to enable GTPase activator activity. Predicted to be involved in activation of GTPase activity.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids D111-E349) was used as the immunogen for the ARHGAP45 antibody.
Limitation:
This ARHGAP45 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q92619