BRDT Antibody / Bromodomain testis-specific protein

NSJ Bioreagents
Product Code: NSJ-RQ7152
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7152-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the BRDT antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human HeLa cells with BRDT antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= BRDT antibody.~
2 / 2
Western blot testing of 1) rat testis and 2) mouse testis tissue with BRDT ant

Flow cytometry testing of human HeLa cells with BRDT antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= BRDT antibody.~
Western blot testing of 1) rat testis and 2) mouse testis tissue with BRDT ant

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the BRDT antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Bromodomain testis-specific protein is a protein that in humans is encoded by the BRDT gene. BRDT is similar to the RING3 protein family. It possesses 2 bromodomain motifs and a PEST sequence (a cluster of proline, glutamic acid, serine, and threonine residues), characteristic of proteins that undergo rapid intracellular degradation. The bromodomain is found in proteins that regulate transcription. Several transcript variants encoding multiple isoforms have been found for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human BRDT protein (amino acids Q795-D947) was used as the immunogen for the BRDT antibody.
Limitation:
This BRDT antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
Q58F21