CDC25B Antibody / M-phase inducer phosphatase 2

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NSJ Bioreagents
Product Code: NSJ-RQ7156
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7156-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the M-phase inducer phosphatase 2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 3
Western blot testing of 1) rat liver, 2) rat brain, 3) rat kidney, 4) rat testis, 5) mouse liver, 6) mouse brain, 7) mouse kidney and 8) mouse testis tissue lysate with M-phase inducer phosphatase 2 antibody. Expected molecular weight: 61~67 kDa (isoforms 1-4).
2 / 3
Western blot testing of human 1) 293T, 2) Caco-2, 3) MOLT4, 4) Raji, 5) HeLa, 6) HaCaT and 7) SiHa cell lysate with M-phase inducer phosphatase 2 antibody. Expected molecular weight: 61~67 kDa (isoforms 1-4).
3 / 3
Flow cytometry testing of human U-87 MG cells with M-phase inducer phosphatase 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= M-phase inducer phosphatase 2 antibody.
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Western blot testing of 1) rat liver, 2) rat brain, 3) rat kidney, 4) rat testis, 5) mouse liver, 6) mouse brain, 7) mouse kidney and 8) mouse testis tissue lysate with M-phase inducer phosphatase 2 antibody. Expected molecular weight: 61~67 kDa (isoforms 1-4).
Western blot testing of human 1) 293T, 2) Caco-2, 3) MOLT4, 4) Raji, 5) HeLa, 6) HaCaT and 7) SiHa cell lysate with M-phase inducer phosphatase 2 antibody. Expected molecular weight: 61~67 kDa (isoforms 1-4).
Flow cytometry testing of human U-87 MG cells with M-phase inducer phosphatase 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= M-phase inducer phosphatase 2 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the M-phase inducer phosphatase 2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Central to the onset of mitosis in all eukaryotic cells is the CDC2 protein kinase, the activity of which is negatively regulated by phosphorylation and positively activated by dephosphorylation. The latter function is carried out by a specific phosphatase, CDC25. At least 3 human CDC25 genes code for the A, B, and C forms of CDC25. CDC25B is mapped to 20p13. P38 kinase has a critical role in the initiation of a G2 delay after ultraviolet radiation. Inhibition of p38 blocks the rapid initiation of this checkpoint in both human and murine cells after ultraviolet radiation. In vitro, p38 binds and phosphorylates CDC25B at serines 309 and 361, and CDC25C at serine-216; phosphorylation of these residues is required for binding to 14-3-3 proteins. In vivo, inhibition of p38 prevents both phosphorylation of CDC25B at serine-309 and 14-3-3 binding after ultraviolet radiation, and mutation of this site is sufficient to inhibit the checkpoint initiation. Regulation of CDC25B phosphorylation by p38 is a critical event for initiating the G2/M checkpoint after ultraviolet radiation.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-H486) was used as the immunogen for the M-phase inducer phosphatase 2 antibody.
Limitation:
This M-phase inducer phosphatase 2 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
P30305