FAA Antibody / FAH / Fumarylacetoacetate hydrolase

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NSJ Bioreagents
Product Code: NSJ-RQ7169
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7169-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Fumarylacetoacetate hydrolase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 5
IHC staining of FFPE human liver cancer tissue with Fumarylacetoacetate hydrolase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE mouse liver tissue with Fumarylacetoacetate hydrolase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE rat liver tissue with Fumarylacetoacetate hydrolase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 5
Western blot testing of 1) human HCCP, 2) human HepG2, 3) rat liver, 4) rat kidney, 5) mouse liver and 6) mouse kidney tissue lysate with Fumarylacetoacetate hydrolase antibody. Predicted molecular weight: 39-46 kDa (two isoforms).
5 / 5
Flow cytometry testing of human PC-3 cells with Fumarylacetoacetate hydrolase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Fumarylacetoacetate hydrolase antibody.
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IHC staining of FFPE human liver cancer tissue with Fumarylacetoacetate hydrolase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse liver tissue with Fumarylacetoacetate hydrolase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat liver tissue with Fumarylacetoacetate hydrolase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HCCP, 2) human HepG2, 3) rat liver, 4) rat kidney, 5) mouse liver and 6) mouse kidney tissue lysate with Fumarylacetoacetate hydrolase antibody. Predicted molecular weight: 39-46 kDa (two isoforms).
Flow cytometry testing of human PC-3 cells with Fumarylacetoacetate hydrolase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Fumarylacetoacetate hydrolase antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Fumarylacetoacetate hydrolase antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Fumarylacetoacetase, also called FAA, Fumarylacetoacetate hydrolase and FAH, is an enzyme that in humans is encoded by the FAH gene located on chromosome 15. The FAH gene is thought to be involved in the catabolism of the amino acid phenylalanine in humans. This gene encodes the last enzyme in the tyrosine catabolism pathway. FAH deficiency is associated with Type 1 hereditary tyrosinemia (HT).
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-P342) was used as the immunogen for the Fumarylacetoacetate hydrolase antibody.
Limitation:
This Fumarylacetoacetate hydrolase antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Uniprot #:
P16930