FKBP1A/B Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ7173
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7173-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the FKBP1A/B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 4
IHC staining of FFPE human rectal cancer tissue with FKBP1A/B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 4
Western blot testing of 1) human HepG2, 2) human MOLT4, 3) human Jurkat, 4) mouse brain and 5) mouse kidney tissue lysate with FKBP1A/B antibody. Predicted molecular weight ~12 kDa.
3 / 4
Flow cytometry testing of human RT4 cells with FKBP1A/B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= FKBP1A/B antibody.
4 / 4
Flow cytometry testing of human SiHa cells with FKBP1A/B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= FKBP1A/B antibody.
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IHC staining of FFPE human rectal cancer tissue with FKBP1A/B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HepG2, 2) human MOLT4, 3) human Jurkat, 4) mouse brain and 5) mouse kidney tissue lysate with FKBP1A/B antibody. Predicted molecular weight ~12 kDa.
Flow cytometry testing of human RT4 cells with FKBP1A/B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= FKBP1A/B antibody.
Flow cytometry testing of human SiHa cells with FKBP1A/B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= FKBP1A/B antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the FKBP1A/B antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Peptidyl-prolyl cis-trans isomerase FKBP1A/1B is an enzyme that in humans is encoded by the FKBP1A/1B gene. The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. The protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin. It interacts with several intracellular signal transduction proteins including type I TGF-beta receptor. It also interacts with multiple intracellular calcium release channels, and coordinates multi-protein complex formation of the tetrameric skeletal muscle ryanodine receptor. In mouse, deletion of this homologous gene causes congenital heart disorder known as noncompaction of left ventricular myocardium. Multiple alternatively spliced variants, encoding the same protein, have been identified. The human genome contains five pseudogenes related to this gene, at least one of which is transcribed.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-H88) was used as the immunogen for the FKBP1A/B antibody.
Limitation:
This FKBP1A/B antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
P62942, P68106