MFF Antibody / Mitochondrial fission factor

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NSJ Bioreagents
Product Code: NSJ-RQ7186
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7186-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Mitochondrial fission factor antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human lymphoma tissue with Mitochondrial fission factor antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
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Immunofluorescent staining of FFPE human SiHa cells with Mitochondrial fission factor antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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IHC staining of FFPE human breast cancer tissue with Mitochondrial fission factor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human rectal cancer tissue with Mitochondrial fission factor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human lymphoma tissue with Mitochondrial fission factor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 9
IHC staining of FFPE mouse brain tissue with Mitochondrial fission factor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE rat brain tissue with Mitochondrial fission factor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 9
Western blot testing of 1) human HeLa, 2) human HepG2, 3) human SiHa, 4) human MOLT-4, 5) human HEL, 6) rat brain and 7) mouse brain tissue lysate with Mitochondrial fission factor antibody. Predicted molecular weight: 25-38 kDa (multiple isoforms).
9 / 9
Flow cytometry testing of human SiHa cells with Mitochondrial fission factor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Mitochondrial fission factor antibody.
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Immunofluorescent staining of FFPE human lymphoma tissue with Mitochondrial fission factor antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human SiHa cells with Mitochondrial fission factor antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human breast cancer tissue with Mitochondrial fission factor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer tissue with Mitochondrial fission factor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lymphoma tissue with Mitochondrial fission factor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with Mitochondrial fission factor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with Mitochondrial fission factor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HeLa, 2) human HepG2, 3) human SiHa, 4) human MOLT-4, 5) human HEL, 6) rat brain and 7) mouse brain tissue lysate with Mitochondrial fission factor antibody. Predicted molecular weight: 25-38 kDa (multiple isoforms).
Flow cytometry testing of human SiHa cells with Mitochondrial fission factor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Mitochondrial fission factor antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Mitochondrial fission factor antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Mitochondrial fission factor (Mff) is a protein that in humans is encoded by the MFF gene. This is a nuclear gene encoding a protein that functions in mitochondrial and peroxisomal fission. The encoded protein recruits dynamin-1-like protein (DNM1L) to mitochondria. There are multiple pseudogenes for this gene on chromosomes 1, 5, and X. Alternative splicing results in multiple transcript variants.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-R342) was used as the immunogen for the Mitochondrial fission factor antibody.
Limitation:
This Mitochondrial fission factor antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q9GZY8