MRE11 Antibody / Meiotic recombination 11

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NSJ Bioreagents
Product Code: NSJ-RQ7189
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7189-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the MRE11 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 7
Flow cytometry testing of human HL60 cells with MRE11 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MRE11 antibody.
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Western blot testing of 1) human A431, 2) human HeLa, 3) human Jurkat, 4) human K562, 5) human Daudi, 6) human HEL, 7) rat brain, 8) rat testis, 9) rat C6, 10) mouse brain, 11) mouse lung, 12) mouse testis and 13) mouse L929 cell lysate with MRE11 antibody. Predicted molecular weight ~81 kDa.
3 / 7
IHC staining of FFPE human tonsil tissue with MRE11 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 7
IHC staining of FFPE human thyroid cancer tissue with MRE11 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 7
IHC staining of FFPE human placental tissue with MRE11 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 7
IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with MRE11 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 7
IHC staining of FFPE human rectal cancer tissue with MRE11 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Flow cytometry testing of human HL60 cells with MRE11 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MRE11 antibody.
Western blot testing of 1) human A431, 2) human HeLa, 3) human Jurkat, 4) human K562, 5) human Daudi, 6) human HEL, 7) rat brain, 8) rat testis, 9) rat C6, 10) mouse brain, 11) mouse lung, 12) mouse testis and 13) mouse L929 cell lysate with MRE11 antibody. Predicted molecular weight ~81 kDa.
IHC staining of FFPE human tonsil tissue with MRE11 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human thyroid cancer tissue with MRE11 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human placental tissue with MRE11 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with MRE11 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer tissue with MRE11 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the MRE11 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
The MRE11 gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids E278-D498) was used as the immunogen for the MRE11 antibody.
Limitation:
This MRE11 antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
P49959