NOS1AP Antibody / CAPON

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NSJ Bioreagents
Product Code: NSJ-RQ7194
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7194-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the NOS1AP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 4
IHC staining of FFPE mouse brain tissue with NOS1AP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE rat brain tissue with NOS1AP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of 1) human HEL, 2) human A431, 3) human HaCaT, 4) human MCF7, 5) rat brain and 6) mouse brain tissue lysate with NOS1AP antibody. Predicted molecular weight ~56 kDa but ca be observed at ~70 kDa (NOS1AP-L) and 95-100 kDa (NOS1APc).
4 / 4
Flow cytometry testing of human HEL cells with NOS1AP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NOS1AP antibody.
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IHC staining of FFPE mouse brain tissue with NOS1AP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with NOS1AP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HEL, 2) human A431, 3) human HaCaT, 4) human MCF7, 5) rat brain and 6) mouse brain tissue lysate with NOS1AP antibody. Predicted molecular weight ~56 kDa but ca be observed at ~70 kDa (NOS1AP-L) and 95-100 kDa (NOS1APc).
Flow cytometry testing of human HEL cells with NOS1AP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NOS1AP antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the NOS1AP antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Nitric oxide synthase 1 adaptor protein (NOS1AP) also known as carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein (CAPON) is a protein that in humans is encoded by the NOS1AP gene. This gene encodes a cytosolic protein that binds to the signaling molecule, neuronal nitric oxide synthase (nNOS). This protein has a C-terminal PDZ-binding domain that mediates interactions with nNOS and an N-terminal phosphotyrosine binding (PTB) domain that binds to the small monomeric G protein, Dexras1. Studies of the related mouse and rat proteins have shown that this protein functions as an adapter protein linking nNOS to specific targets, such as Dexras1 and the synapsins. Alternative splicing results in multiple transcript variants encoding different isoforms
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids D24-D438) was used as the immunogen for the NOS1AP antibody.
Limitation:
This NOS1AP antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
O75052