PRKAG2 Antibody / AMPK gamma 2

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NSJ Bioreagents
Product Code: NSJ-RQ7196
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7196-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the AMPK gamma 2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Western blot testing of 1) human 293T, 2) human HeLa, 3) human placenta, 4) human U-87 MG, 5) human A431, 6) rat heart and 7) mouse NIH 3T3 cell lysate with AMPK gamma 2 antibody. Predicted molecular weight ~63 kDa.~
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Flow cytometry testing of human
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Western blot testing of 1) human 293T, 2) human HeLa, 3) human placenta, 4) human U-87 MG, 5) human A431, 6) rat heart and 7) mouse NIH 3T3 cell lysate with AMPK gamma 2 antibody. Predicted molecular weight ~63 kDa.~
Flow cytometry testing of human

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the AMPK gamma 2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
5'-AMP-activated protein kinase subunit gamma-2 is an enzyme that in humans is encoded by the PRKAG2 gene. AMP-activated protein kinase (AMPK) is a heterotrimeric protein composed of a catalytic alpha subunit, a noncatalytic beta subunit, and a noncatalytic regulatory gamma subunit. Various forms of each of these subunits exist, encoded by different genes. AMPK is an important energy-sensing enzyme that monitors cellular energy status and functions by inactivating key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This gene is a member of the AMPK gamma subunit family. Mutations in this gene have been associated with Wolff-Parkinson-White syndrome, familial hypertrophic cardiomyopathy, and glycogen storage disease of the heart. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids K24-H530) was used as the immunogen for the AMPK gamma 2 antibody.
Limitation:
This AMPK gamma 2 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
Q9UGJ0