SNAIL Antibody / SNAI1

NSJ Bioreagents
Product Code: NSJ-RQ7205
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7205-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the SNAI1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 3
Western blot testing of 1) rat RH35, 2) mouse liver, 3) mouse lung, 4) mouse heart and 5) mouse HEPA1-6 cell lysate with SNAI1 antibody. Predicted molecular weight ~29 kDa.
2 / 3
Western blot testing of human 1) A431, 2) HepG2, 3) A549, 4) PC-3, 5) K562, 6) A431, 7) SW620 and 8) Raji cell lysate with SNAI1 antibody. Predicted molecular weight ~29 kDa.
3 / 3
Flow cytometry testing of human 293T cells with SNAI1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SNAI1 antibody.

Western blot testing of 1) rat RH35, 2) mouse liver, 3) mouse lung, 4) mouse heart and 5) mouse HEPA1-6 cell lysate with SNAI1 antibody. Predicted molecular weight ~29 kDa.
Western blot testing of human 1) A431, 2) HepG2, 3) A549, 4) PC-3, 5) K562, 6) A431, 7) SW620 and 8) Raji cell lysate with SNAI1 antibody. Predicted molecular weight ~29 kDa.
Flow cytometry testing of human 293T cells with SNAI1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SNAI1 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the SNAI1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
The Drosophila embryonic protein SNAI1, commonly known as Snail, is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. And it is located in 16q24.3. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2. It is studied that SNAIL gene may show a role in recurrence of breast cancer by downregulating E-cadherin and inducing anepithelial to mesenchymal transition.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-K170) was used as the immunogen for the SNAI1 antibody.
Limitation:
This SNAI1 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
O95863