TRPV6 Antibody / CaT-L / CaT1

NSJ Bioreagents
Product Code: NSJ-RQ7210
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7210-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the TRPV6 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human RT4 cells with TRPV6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TRPV6 antibody.~
2 / 2
Western blot testing of human 1) placenta, 2) PC-3, 3) HepG2 and 4) HGC-27 ce

Flow cytometry testing of human RT4 cells with TRPV6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TRPV6 antibody.~
Western blot testing of human 1) placenta, 2) PC-3, 3) HepG2 and 4) HGC-27 ce

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the TRPV6 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
TRPV6 (Transient Receptor Potential Cation Channel Subfamily V Member 6), also known as ECAC2, CAT1 or CATL, a membrane calcium channel which is responsible for the first step in calcium absorption in the intestine. TRPV6 is a member of the Transient receptor potential (TRP) family of membrane proteins. Using FISH, Peng et al.(2000) mapped the TRPV6 gene to chromosome 7q33-q34. Peng et al.(2000) determined that TRPV6 mediates calcium uptake independent of sodium, chloride, and hydrogen; however, calcium influx was higher at alkaline rather than acidic pH. Functional analysis by Peng et al.(2001) indicated that TRPV5 mediates greater calcium uptake than TRPV6. They concluded that TRPV5 and TRPV6 are calcium-selective channels and share a common ancestral gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids A15-I765) was used as the immunogen for the TRPV6 antibody.
Limitation:
This TRPV6 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
Q9H1D0