ATP5A1 Antibody / ATP5F1A / ATP synthase subunit alpha

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NSJ Bioreagents
Product Code: NSJ-RQ7217
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7217-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the ATP5A1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 10
Immunofluorescent staining of FFPE human placental tissue with ATP5A1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
2 / 10
Immunofluorescent staining of FFPE human colon cancer tissue with ATP5A1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
3 / 10
Immunofluorescent staining of FFPE human HepG2 cells with ATP5A1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
4 / 10
IHC staining of FFPE human endometrial adenocarcinoma tissue with ATP5A1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 10
IHC staining of FFPE human thyroid cancer tissue with ATP5A1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 10
IHC staining of FFPE human rectal cancer tissue with ATP5A1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 10
IHC staining of FFPE human placental tissue with ATP5A1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 10
Western blot testing of human 1) SK-OV-3, 2) MCF7, 3) HL60, 4) RT4, 5) Daudi, 6) ThP-1 and 7) HepG2 cell lysate with ATP5A1 antibody. Predicted molecular weight ~54-60 kDa (multiple isoforms).
9 / 10
Western blot testing of 1) rat heart, 2) rat brain, 3) rat lung, 4) rat H9C2(2-1), 5) mouse heart, 6) mouse brain, 7) mouse lung and 8) mouse NIH 3T3 cell lysate with ATP5A1 antibody. Predicted molecular weight ~54-60 kDa (multiple isoforms).
10 / 10
Flow cytometry testing of human U937 cells with ATP5A1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATP5A1 antibody.
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Immunofluorescent staining of FFPE human placental tissue with ATP5A1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
Immunofluorescent staining of FFPE human colon cancer tissue with ATP5A1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
Immunofluorescent staining of FFPE human HepG2 cells with ATP5A1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human endometrial adenocarcinoma tissue with ATP5A1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human thyroid cancer tissue with ATP5A1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer tissue with ATP5A1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human placental tissue with ATP5A1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) SK-OV-3, 2) MCF7, 3) HL60, 4) RT4, 5) Daudi, 6) ThP-1 and 7) HepG2 cell lysate with ATP5A1 antibody. Predicted molecular weight ~54-60 kDa (multiple isoforms).
Western blot testing of 1) rat heart, 2) rat brain, 3) rat lung, 4) rat H9C2(2-1), 5) mouse heart, 6) mouse brain, 7) mouse lung and 8) mouse NIH 3T3 cell lysate with ATP5A1 antibody. Predicted molecular weight ~54-60 kDa (multiple isoforms).
Flow cytometry testing of human U937 cells with ATP5A1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATP5A1 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the ATP5A1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
ATP synthase F1 subunit alpha, mitochondrial, also called ATP synthase subunit alpha and ATP5A1, is an enzyme that in humans is encoded by the ATP5F1A gene. This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids R12-Q520) was used as the immunogen for the ATP5A1 antibody.
Limitation:
This ATP5A1 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
P25705