ATP5B Antibody / ATP5F1B / ATP synthase subunit beta

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NSJ Bioreagents
Product Code: NSJ-RQ7218
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7218-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the ATP5F1B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 14
IHC staining of FFPE human breast cancer with ATP5F1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
2 / 14
IHC staining of FFPE human ovarian cancer with ATP5F1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
3 / 14
IHC staining of FFPE human placental tissue with ATP5F1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
4 / 14
IHC staining of FFPE human esophageal squamous carcinoma tissue with ATP5F1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
5 / 14
IHC staining of FFPE human endometrial adenocarcinoma tissue with ATP5F1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
6 / 14
IHC staining of FFPE human rectal cancer tissue with ATP5F1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
7 / 14
IHC staining of FFPE mouse heart tissue with ATP5F1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
8 / 14
IHC staining of FFPE rat heart tissue with ATP5F1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
9 / 14
Immunofluorescent staining of FFPE human ovarian cancer tissue with ATP5F1B antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
10 / 14
Immunofluorescent staining of FFPE human breast cancer tissue with ATP5F1B antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
11 / 14
Immunofluorescent staining of FFPE human PC-3 cells with ATP5F1B antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
12 / 14
Western blot testing of human 1) HepG2, 2) HeLa, 3) PC-3, 4) HL60, 5) T-47D, 6) Caco-2, 7) HEL and 8) K562 cell lysate with ATP5F1B antibody. Predicted molecular weight ~56 kDa.
13 / 14
Western blot testing of 1) rat heart, 2) rat liver, 3) rat lung, 4) rat RH35, 5) mouse heart, 6) mouse liver, 7) mouse lung and 8) mouse RAW264.7 cell lysate with ATP5F1B antibody. Predicted molecular weight ~56 kDa.
14 / 14
Flow cytometry testing of human 293T cells with ATP5F1B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATP5F1B antibody.
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IHC staining of FFPE human breast cancer with ATP5F1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian cancer with ATP5F1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human placental tissue with ATP5F1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human esophageal squamous carcinoma tissue with ATP5F1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human endometrial adenocarcinoma tissue with ATP5F1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer tissue with ATP5F1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE mouse heart tissue with ATP5F1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE rat heart tissue with ATP5F1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human ovarian cancer tissue with ATP5F1B antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
Immunofluorescent staining of FFPE human breast cancer tissue with ATP5F1B antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
Immunofluorescent staining of FFPE human PC-3 cells with ATP5F1B antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HepG2, 2) HeLa, 3) PC-3, 4) HL60, 5) T-47D, 6) Caco-2, 7) HEL and 8) K562 cell lysate with ATP5F1B antibody. Predicted molecular weight ~56 kDa.
Western blot testing of 1) rat heart, 2) rat liver, 3) rat lung, 4) rat RH35, 5) mouse heart, 6) mouse liver, 7) mouse lung and 8) mouse RAW264.7 cell lysate with ATP5F1B antibody. Predicted molecular weight ~56 kDa.
Flow cytometry testing of human 293T cells with ATP5F1B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATP5F1B antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the ATP5F1B antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids Q123-S529) was used as the immunogen for the ATP5F1B antibody.
Limitation:
This ATP5F1B antibody is available for research use only.
Localization:
Cytoplasmic (mitochondria)
Purity:
Antigen affinity purified
Uniprot #:
P06576