CD135 Antibody / FLT3

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NSJ Bioreagents
Product Code: NSJ-RQ7227
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7227-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the CD135 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human spleen tissue with CD135 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.~
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Western blot testing of human HCCP cell lysate with CD135 antibody. Expected molecular weight: 113-160
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IHC staining of FFPE human spleen tissue with CD135 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.~
Western blot testing of human HCCP cell lysate with CD135 antibody. Expected molecular weight: 113-160

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the CD135 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Cluster of differentiation antigen 135 (CD135) also known as fms like tyrosine kinase 3 (FLT-3), receptor-type tyrosine-protein kinase FLT3, or fetal liver kinase-2 (Flk2) is a protein that in humans is encoded by the FLT3 gene. This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. This receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids A66-S993) was used as the immunogen for the CD135 antibody.
Limitation:
This CD135 antibody is available for research use only.
Localization:
Cell membrane, cytoplasm
Purity:
Antigen affinity purified
Uniprot #:
P36888