NDUFS6 Antibody / NADH-ubiquinone oxidoreductase 13 kDa-A subunit

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NSJ Bioreagents
Product Code: NSJ-RQ7235
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7235-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the NDUFS6 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Western blot testing of 1) rat brain, 2) rat heart, 3) rat kidney, 4) rat liver, 5) mouse brain, 6) mouse heart, 7) mouse kidney and 8) mouse liver tissue lysate with NDUFS6 antibody. Expected molecular weight ~13 kDa.
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Western blot testing of human 1) HepG2, 2) HUH-7, 3) HCCT and 4) HCCP cell lysate with NDUFS6 antibody. Expected molecular weight ~13 kDa.
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IHC staining of FFPE human ovarian cancer tissue with NDUFS6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 6
IHC staining of FFPE human breast cancer tissue with NDUFS6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 6
IHC staining of FFPE mouse heart tissue with NDUFS6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 6
Flow cytometry testing of human HeLa cells with NDUFS6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NDUFS6 antibody.
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Western blot testing of 1) rat brain, 2) rat heart, 3) rat kidney, 4) rat liver, 5) mouse brain, 6) mouse heart, 7) mouse kidney and 8) mouse liver tissue lysate with NDUFS6 antibody. Expected molecular weight ~13 kDa.
Western blot testing of human 1) HepG2, 2) HUH-7, 3) HCCT and 4) HCCP cell lysate with NDUFS6 antibody. Expected molecular weight ~13 kDa.
IHC staining of FFPE human ovarian cancer tissue with NDUFS6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer tissue with NDUFS6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse heart tissue with NDUFS6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Flow cytometry testing of human HeLa cells with NDUFS6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NDUFS6 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the NDUFS6 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial is an enzyme that in humans is encoded by the NDUFS6 gene. This gene encodes a subunit of the NADH:ubiquinone oxidoreductase (complex I), which is the first enzyme complex in the electron transport chain of mitochondria. This complex functions in the transfer of electrons from NADH to the respiratory chain. The subunit encoded by this gene is one of seven subunits in the iron-sulfur protein fraction. Mutations in this gene cause mitochondrial complex I deficiency, a disease that causes a wide variety of clinical disorders, including neonatal disease and adult-onset neurodegenerative disorders.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-D88) was used as the immunogen for the NDUFS6 antibody.
Limitation:
This NDUFS6 antibody is available for research use only.
Localization:
Cytoplasmic (mitochondria)
Purity:
Antigen affinity purified
Uniprot #:
O75380