NDUFS8 Antibody / NADH-ubiquinone oxidoreductase 23 kDa subunit

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NSJ Bioreagents
Product Code: NSJ-RQ7236
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7236-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the NDUFS8 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Western blot testing of 1) rat heart, 2) rat liver, 3) rat skeletal muscle, 4) mouse heart, 5) mouse liver, 6) mouse skeletal muscle and 7) mouse C2C12 cell lysate with NDUFS8 antibody. Expected molecular weight ~23 kDa.
2 / 7
Western blot testing of human 1) HeLa, 2) HepG2, 3) A549, 4) MCF7, 5) Caco-2, 6) PC-3 and 7) 293T cell lysate with NDUFS8 antibody. Expected molecular weight ~23 kDa.
3 / 7
IHC staining of FFPE human liver cancer tissue with NDUFS8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 7
IHC staining of FFPE mouse heart tissue with NDUFS8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 7
Immunofluorescent staining of FFPE human liver cancer tissue with NDUFS8 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
6 / 7
Flow cytometry testing of human 293T cells with NDUFS8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NDUFS8 antibody.
7 / 7
Flow cytometry testing of human HeLa cells with NDUFS8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NDUFS8 antibody.
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Western blot testing of 1) rat heart, 2) rat liver, 3) rat skeletal muscle, 4) mouse heart, 5) mouse liver, 6) mouse skeletal muscle and 7) mouse C2C12 cell lysate with NDUFS8 antibody. Expected molecular weight ~23 kDa.
Western blot testing of human 1) HeLa, 2) HepG2, 3) A549, 4) MCF7, 5) Caco-2, 6) PC-3 and 7) 293T cell lysate with NDUFS8 antibody. Expected molecular weight ~23 kDa.
IHC staining of FFPE human liver cancer tissue with NDUFS8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse heart tissue with NDUFS8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human liver cancer tissue with NDUFS8 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Flow cytometry testing of human 293T cells with NDUFS8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NDUFS8 antibody.
Flow cytometry testing of human HeLa cells with NDUFS8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NDUFS8 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the NDUFS8 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial also known as NADH-ubiquinone oxidoreductase 23 kDa subunit, Complex I-23kD (CI-23kD), or TYKY subunit is an enzyme that in humans is encoded by the NDUFS8 gene. This gene encodes a subunit of mitochondrial NADH:ubiquinone oxidoreductase, or Complex I, a multimeric enzyme of the respiratory chain responsible for NADH oxidation, ubiquinone reduction, and the ejection of protons from mitochondria. The encoded protein is involved in the binding of two of the six to eight iron-sulfur clusters of Complex I and, as such, is required in the electron transfer process. Mutations in this gene have been associated with Leigh syndrome.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-R210) was used as the immunogen for the NDUFS8 antibody.
Limitation:
This NDUFS8 antibody is available for research use only.
Localization:
Cytoplasmic (mitochondria)
Purity:
Antigen affinity purified
Uniprot #:
O00217