NOP58 Antibody / Nucleolar protein 58

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NSJ Bioreagents
Product Code: NSJ-RQ7238
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7238-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the NOP58 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 4
Immunofluorescent staining of FFPE human Caco-2 cells with NOP58 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
Western blot testing of human 1) HeLa, 2) K562, 3) HEL, 4) MOLT4, 5) U-251 and 6) HepG2 cell lysate with NOP58 antibody. Predicted molecular weight ~60 kDa, commonly observed at 58-65 kDa.
3 / 4
Flow cytometry testing of human U-251 cells with NOP58 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NOP58 antibody.
4 / 4
Flow cytometry testing of human U937 cells with NOP58 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NOP58 antibody.
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Immunofluorescent staining of FFPE human Caco-2 cells with NOP58 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HeLa, 2) K562, 3) HEL, 4) MOLT4, 5) U-251 and 6) HepG2 cell lysate with NOP58 antibody. Predicted molecular weight ~60 kDa, commonly observed at 58-65 kDa.
Flow cytometry testing of human U-251 cells with NOP58 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NOP58 antibody.
Flow cytometry testing of human U937 cells with NOP58 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NOP58 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the NOP58 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Nucleolar protein 58 is a protein that in humans is encoded by the NOP58 gene. The protein encoded by this gene is a core component of box C/D small nucleolar ribonucleoproteins. Some box C/D small nucleolar RNAs (snoRNAs), such as U3, U8, and U14, are dependent upon the encoded protein for their synthesis. This protein is SUMOylated, which is necessary for high affinity binding to snoRNAs.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids K30-K450) was used as the immunogen for the NOP58 antibody.
Limitation:
This NOP58 antibody is available for research use only.
Localization:
Nucleus, Nucleolus
Purity:
Antigen affinity purified
Uniprot #:
Q9Y2X3