RPL36 Antibody / 60S ribosomal protein L36

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NSJ Bioreagents
Product Code: NSJ-RQ7243
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7243-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the RPL36 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 10
Western blot testing of 1) rat brain, 2) rat stomach, 3) rat liver, 4) rat PC-12, 5) mouse brain, 6) mouse liver and 7) mouse NIH 3T3 cell lysate with RPL36 antibody. Predicted molecular weight ~12 kDa.
2 / 10
Western blot testing of human 1) HeLa, 2) 293T, 3) Jurkat, 4) PC-3, 5) HepG2, 6) ThP-1, 7) U937 and 8) monkey COS-7 cell lysate with RPL36 antibody. Predicted molecular weight ~12 kDa.
3 / 10
IHC staining of FFPE human ovarian cancer tissue with RPL36 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 10
IHC staining of FFPE human endometrial adenocarcinoma tissue with RPL36 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 10
IHC staining of FFPE human breast cancer tissue with RPL36 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 10
IHC staining of FFPE human cervical cancer tissue with RPL36 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 10
IHC staining of FFPE rat colon tissue with RPL36 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 10
Immunofluorescent staining of FFPE human endometrial cancer tissue with RPL36 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
9 / 10
Immunofluorescent staining of FFPE human colon cancer tissue with RPL36 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
10 / 10
Flow cytometry testing of human HEL cells with RPL36 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RPL36 antibody.
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Western blot testing of 1) rat brain, 2) rat stomach, 3) rat liver, 4) rat PC-12, 5) mouse brain, 6) mouse liver and 7) mouse NIH 3T3 cell lysate with RPL36 antibody. Predicted molecular weight ~12 kDa.
Western blot testing of human 1) HeLa, 2) 293T, 3) Jurkat, 4) PC-3, 5) HepG2, 6) ThP-1, 7) U937 and 8) monkey COS-7 cell lysate with RPL36 antibody. Predicted molecular weight ~12 kDa.
IHC staining of FFPE human ovarian cancer tissue with RPL36 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human endometrial adenocarcinoma tissue with RPL36 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer tissue with RPL36 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human cervical cancer tissue with RPL36 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat colon tissue with RPL36 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human endometrial cancer tissue with RPL36 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human colon cancer tissue with RPL36 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Flow cytometry testing of human HEL cells with RPL36 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RPL36 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 5ug/ml
Application Note:
Optimal dilution of the RPL36 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
60S ribosomal protein L36 is a protein that in humans is encoded by the RPL36 gene. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L36E family of ribosomal proteins. It is located in the cytoplasm. Transcript variants derived from alternative splicing exist; they encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids REELSNVLAAMRKAAAKKD were used as the immunogen for the RPL36 antibody.
Limitation:
This RPL36 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q9Y3U8