RRS1 Antibody / Ribosome biogenesis regulatory protein homolog

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NSJ Bioreagents
Product Code: NSJ-RQ7244
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7244-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the RRS1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 9
Immunofluorescent staining of FFPE human PC-3 cells with RRS1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 9
Western blot testing of human 1) PC-3, 2) HeLa, 3) MCF7, 4) K562, 5) 293T, 6) U-251, 7) Daudi and 8) MOLT4 cell lysate with RRS1 antibody. Predicted molecular weight ~41 kDa.
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IHC staining of FFPE human ovarian cancer tissue with RRS1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 9
IHC staining of FFPE human colorectal adenocarcinoma tissue with RRS1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 9
IHC staining of FFPE human esophageal squamous carcinoma tissue with RRS1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 9
IHC staining of FFPE human liver cancer tissue with RRS1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 9
IHC staining of FFPE human cervical cancer tissue with RRS1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 9
Flow cytometry testing of human HEL cells with RRS1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RRS1 antibody.
9 / 9
Immunofluorescent staining of FFPE human colon cancer tissue with RRS1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human PC-3 cells with RRS1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) PC-3, 2) HeLa, 3) MCF7, 4) K562, 5) 293T, 6) U-251, 7) Daudi and 8) MOLT4 cell lysate with RRS1 antibody. Predicted molecular weight ~41 kDa.
IHC staining of FFPE human ovarian cancer tissue with RRS1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colorectal adenocarcinoma tissue with RRS1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human esophageal squamous carcinoma tissue with RRS1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with RRS1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human cervical cancer tissue with RRS1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Flow cytometry testing of human HEL cells with RRS1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RRS1 antibody.
Immunofluorescent staining of FFPE human colon cancer tissue with RRS1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the RRS1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Ribosome biogenesis regulatory protein homolog is a protein that in humans is encoded by the RRS1 gene. RRS1 involved in ribosomal large subunit assembly and may regulate the localization of the 5S RNP/5S ribonucleoprotein particle to the nucleolus.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids ELLRVMNSKKPQLDVTRATNKQ were used as the immunogen for the RRS1 antibody.
Limitation:
This RRS1 antibody is available for research use only.
Localization:
Nuclear, nucleolus
Purity:
Antigen affinity purified
Uniprot #:
Q15050