RuvB-like 1 Antibody / RUVBL1 / TIP49A

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NSJ Bioreagents
Product Code: NSJ-RQ7245
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7245-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Monkey
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the TIP49A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 10
IHC staining of FFPE human ovarian cancer tissue with TIP49A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 10
IHC staining of FFPE human spleen tissue with TIP49A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 10
IHC staining of FFPE human tonsil tissue with TIP49A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 10
IHC staining of FFPE human thyroid cancer tissue with TIP49A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 10
IHC staining of FFPE human lung adenocarcinoma tissue with TIP49A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 10
IHC staining of FFPE human colorectal adenocarcinoma tissue with TIP49A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 10
Immunofluorescent staining of FFPE human A549 cells with TIP49A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
8 / 10
Western blot testing of 1) human K562, 2) human 293T, 3) human HT1080, 4) monkey COS-7, 5) human MCF7, 6) human Daudi, 7) human MOLT4, 8) human HEL, 9) rat testis, 10) rat C6, 11) mouse testis and 12) mouse NIH 3T3 cell lysate with TIP49A antibody. Predicted molecular weight ~50 kDa.
9 / 10
Flow cytometry testing of human HeLa cells with TIP49A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TIP49A antibody.
10 / 10
Immunofluorescent staining of FFPE human colon cancer tissue with TIP49A antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
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IHC staining of FFPE human ovarian cancer tissue with TIP49A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human spleen tissue with TIP49A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with TIP49A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human thyroid cancer tissue with TIP49A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung adenocarcinoma tissue with TIP49A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colorectal adenocarcinoma tissue with TIP49A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human A549 cells with TIP49A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human K562, 2) human 293T, 3) human HT1080, 4) monkey COS-7, 5) human MCF7, 6) human Daudi, 7) human MOLT4, 8) human HEL, 9) rat testis, 10) rat C6, 11) mouse testis and 12) mouse NIH 3T3 cell lysate with TIP49A antibody. Predicted molecular weight ~50 kDa.
Flow cytometry testing of human HeLa cells with TIP49A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TIP49A antibody.
Immunofluorescent staining of FFPE human colon cancer tissue with TIP49A antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the TIP49A antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
RuvB-like 1 (E. coli), also known as RUVBL1 and TIP49, is a human gene. This gene encodes a protein that has both DNA-dependent ATPase and DNA helicase activities and belongs to the ATPases associated with diverse cellular activities (AAA+) protein family. The encoded protein associates with several multisubunit transcriptional complexes and with protein complexes involved in both ATP-dependent remodeling and histone modification. Alternate splicing results in multiple transcript variants.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids Q13-D23) was used as the immunogen for the TIP49A antibody.
Limitation:
This TIP49A antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q9Y265