SEPT2 Antibody / Septin 2

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NSJ Bioreagents
Product Code: NSJ-RQ7247
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7247-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Monkey
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Septin 2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 11
Immunofluorescent staining of FFPE mouse brain tissue with Septin 2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
2 / 11
Immunofluorescent staining of FFPE rat brain tissue with Septin 2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
3 / 11
Immunofluorescent staining of FFPE human endometrial cancer tissue with Septin 2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
4 / 11
Immunofluorescent staining of FFPE human SiHa cells with Septin 2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
5 / 11
IHC staining of FFPE mouse brain tissue with Septin 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 11
IHC staining of FFPE mouse brain tissue with Septin 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 11
IHC staining of FFPE rat brain tissue with Septin 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 11
IHC staining of FFPE human colorectal adenocarcinoma tissue with Septin 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
9 / 11
Western blot testing of 1) human MCF7, 2) human U-87 MG, 3) human HeLa, 4) human K562, 5) monkey COS-7, 6) human HepG2 and 7) human HEL cell lysate with Septin 2 antibody. Predicted molecular weight: ~41 kDa.
10 / 11
Western blot testing of 1) rat brain, 2) rat kidney, 3) rat testis, 4) rat C6, 5) mouse brain, 6) mouse testis and 7) mouse Neuro-2a cell lysate with Septin 2 antibody. Predicted molecular weight: ~41 kDa.
11 / 11
Flow cytometry testing of human U-251 cells with Septin 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Septin 2 antibody.
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Immunofluorescent staining of FFPE mouse brain tissue with Septin 2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE rat brain tissue with Septin 2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human endometrial cancer tissue with Septin 2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human SiHa cells with Septin 2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE mouse brain tissue with Septin 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with Septin 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with Septin 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colorectal adenocarcinoma tissue with Septin 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human MCF7, 2) human U-87 MG, 3) human HeLa, 4) human K562, 5) monkey COS-7, 6) human HepG2 and 7) human HEL cell lysate with Septin 2 antibody. Predicted molecular weight: ~41 kDa.
Western blot testing of 1) rat brain, 2) rat kidney, 3) rat testis, 4) rat C6, 5) mouse brain, 6) mouse testis and 7) mouse Neuro-2a cell lysate with Septin 2 antibody. Predicted molecular weight: ~41 kDa.
Flow cytometry testing of human U-251 cells with Septin 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Septin 2 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Septin 2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Septin 2, also known as SEPT2, is a protein which in humans is encoded by the SEPT2 gene. Enables identical protein binding activity. Predicted to be involved in several processes, including cilium assembly; regulation of exocytosis; and smoothened signaling pathway. Predicted to act upstream of or within regulation of L-glutamate import across plasma membrane and regulation of protein localization. Located in several cellular components, including cytoskeleton; photoreceptor connecting cilium; and sperm annulus. Part of septin complex.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-E326) was used as the immunogen for the Septin 2 antibody.
Limitation:
This Septin 2 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q15019