SEPT3 Antibody / Septin 3

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NSJ Bioreagents
Product Code: NSJ-RQ7248
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7248-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the SEPT3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 5
IHC staining of FFPE mouse brain tissue with SEPT3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE rat brain tissue with SEPT3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
Immunofluorescent staining of FFPE human SiHa cells with SEPT3 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
4 / 5
Western blot testing of 1) human Daudi, 2) human SiHa, 3) rat brain and 4) mouse brain tissue lysate with SEPT3 antibody. Predicted molecular weight ~40 kDa.
5 / 5
Flow cytometry testing of human U-251 cells with SEPT3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SEPT3 antibody.
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IHC staining of FFPE mouse brain tissue with SEPT3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with SEPT3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human SiHa cells with SEPT3 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human Daudi, 2) human SiHa, 3) rat brain and 4) mouse brain tissue lysate with SEPT3 antibody. Predicted molecular weight ~40 kDa.
Flow cytometry testing of human U-251 cells with SEPT3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SEPT3 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the SEPT3 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
This gene belongs to the septin family of GTPases. Members of this family are required for cytokinesis. Expression is upregulated by retinoic acid in a human teratocarcinoma cell line. The specific function of this gene has not been determined. Alternative splicing of this gene results in several transcript variants encoding different isoforms.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-E339) was used as the immunogen for the SEPT3 antibody.
Limitation:
This SEPT3 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
Q9UH03