SBDS Antibody / Shwachman-Bodian-Diamond syndrome protein

Contact us
NSJ Bioreagents
Product Code: NSJ-RQ7423
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7423-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the SBDS antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 3
Flow cytometry testing of human HL60 cells with SBDS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SBDS antibody.
2 / 3
Flow cytometry testing of human Caco-2 cells with SBDS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SBDS antibody.
3 / 3
Western blot testing of human 1) 293T, 2) HepG2 and 3) HL60 cell lysate with SBDS antibody. Predicted molecular weight ~29 kDa.
❮❯

Flow cytometry testing of human HL60 cells with SBDS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SBDS antibody.
Flow cytometry testing of human Caco-2 cells with SBDS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SBDS antibody.
Western blot testing of human 1) 293T, 2) HepG2 and 3) HL60 cell lysate with SBDS antibody. Predicted molecular weight ~29 kDa.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the SBDS antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Ribosome maturation protein SBDS is a protein that in humans is encoded by the SBDS gene. This gene encodes a highly conserved protein that plays an essential role in ribosome biogenesis. The encoded protein interacts with elongation factor-like GTPase 1 to disassociate eukaryotic initiation factor 6 from the late cytoplasmic pre-60S ribosomal subunit allowing assembly of the 80S subunit. Mutations within this gene are associated with the autosomal recessive disorder Shwachman-Bodian-Diamond syndrome. This gene has a closely linked pseudogene that is distally located.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids R19-D241) was used as the immunogen for the SBDS antibody.
Limitation:
This SBDS antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
Q9Y3A5