UBE2N Antibody / Ubc13

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NSJ Bioreagents
Product Code: NSJ-RQ7424
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7424-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the UBE2N antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 7
IHC staining of FFPE human cervical cancer tissue with UBE2N antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human lung squamous cell carcinoma tissue with UBE2N antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 7
IHC staining of FFPE human spleen tissue with UBE2N antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 7
IHC staining of FFPE human breast cancer tissue with UBE2N antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 7
Immunofluorescent staining of FFPE human A549 cells with GNB1 antibody. HIER: steam section in pH6 citrate buffer for 20 min.
6 / 7
Western blot testing of 1) human Daudi, 2) human MOLT4, 3) human RT4, 4) human HEL, 5) rat heart, 6) rat thymus, 7) rat C6, 8) mouse thymus, 9) mouse small intestine and 10) mouse NIH 3T3 cell lysate with UBE2N antibody. Predicted molecular weight ~17 kDa.
7 / 7
Flow cytometry testing of human Daudi cells with UBE2N antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= UBE2N antibody.
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IHC staining of FFPE human cervical cancer tissue with UBE2N antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung squamous cell carcinoma tissue with UBE2N antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human spleen tissue with UBE2N antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer tissue with UBE2N antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human A549 cells with GNB1 antibody. HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human Daudi, 2) human MOLT4, 3) human RT4, 4) human HEL, 5) rat heart, 6) rat thymus, 7) rat C6, 8) mouse thymus, 9) mouse small intestine and 10) mouse NIH 3T3 cell lysate with UBE2N antibody. Predicted molecular weight ~17 kDa.
Flow cytometry testing of human Daudi cells with UBE2N antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= UBE2N antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Immunohistochemistry (FFPE): 2-5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the UBE2N antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Ubiquitin-conjugating enzyme E2 N is a protein that in humans is encoded by the UBE2N gene. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids Q13-I152) was used as the immunogen for the UBE2N antibody.
Limitation:
This UBE2N antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
P61088