WNT10B Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ7426
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7426-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the WNT10B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human PC-3 cells with WNT10B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= WNT10B antibody.
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Flow cytometry testing of mouse ANA-1 cells with WNT10B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= WNT10B antibody.
3 / 4
Flow cytometry testing of rat NRK cells with WNT10B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= WNT10B antibody.
4 / 4
Western blot testing of human 1) T-47D and 2) HeLa cell lysate with WNT10B antibody. Predicted molecular weight ~45 kDa.
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Flow cytometry testing of human PC-3 cells with WNT10B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= WNT10B antibody.
Flow cytometry testing of mouse ANA-1 cells with WNT10B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= WNT10B antibody.
Flow cytometry testing of rat NRK cells with WNT10B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= WNT10B antibody.
Western blot testing of human 1) T-47D and 2) HeLa cell lysate with WNT10B antibody. Predicted molecular weight ~45 kDa.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the WNT10B antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Protein Wnt-10b (formerly Wnt12[5]) is a protein that in humans is encoded by the WNT10B gene. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It may be involved in breast cancer, and its protein signaling is likely a molecular switch that governs adipogenesis. This protein is 96% identical to the mouse Wnt10b protein at the amino acid level. This gene is clustered with another family member, WNT1, in the chromosome 12q13 region.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids RLPHHSAILKRGFRE from the human protein were used as the immunogen for the WNT10B antibody.
Limitation:
This WNT10B antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
O00744