ZNRF3 Antibody / Zinc/RING finger protein 3

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NSJ Bioreagents
Product Code: NSJ-RQ7436
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7436-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the ZNRF3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human U-2 OS cells with ZNRF3 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human 1) Caco-2, 2) A549 and 3) HaCaT cell lysate with ZNRF3 antibody. Predicted molecular weight ~101 kDa.
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Flow cytometry testing of human PC-3 cells with ZNRF3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ZNRF3 antibody.
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Immunofluorescent staining of FFPE human U-2 OS cells with ZNRF3 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) Caco-2, 2) A549 and 3) HaCaT cell lysate with ZNRF3 antibody. Predicted molecular weight ~101 kDa.
Flow cytometry testing of human PC-3 cells with ZNRF3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ZNRF3 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the ZNRF3 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Enables frizzled binding activity and ubiquitin-protein transferase activity. Involved in cellular protein metabolic process and negative regulation of Wnt signaling pathway. Is integral component of plasma membrane.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids R54-E913) was used as the immunogen for the ZNRF3 antibody.
Limitation:
This ZNRF3 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
Q9ULT6