UBE2S Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ7437
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7437-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the UBE2S antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 3
Immunofluorescent staining of FFPE human A549 cells with UBE2S antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human 293T, 2) human HeLa, 3) human Jurkat, 4) human MCF-7 and 5) rat testis tissue lysate with UBE2S antibody. Predicted molecular weight ~24 kDa.
3 / 3
Flow cytometry testing of human HL60 cells with UBE2S antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= UBE2S antibody.
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Immunofluorescent staining of FFPE human A549 cells with UBE2S antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human 293T, 2) human HeLa, 3) human Jurkat, 4) human MCF-7 and 5) rat testis tissue lysate with UBE2S antibody. Predicted molecular weight ~24 kDa.
Flow cytometry testing of human HL60 cells with UBE2S antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= UBE2S antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the UBE2S antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids E6-H155) was used as the immunogen for the UBE2S antibody.
Limitation:
This UBE2S antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q16763