TPM2 Antibody / Tropomyosin 2 / Tropomyosin beta

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NSJ Bioreagents
Product Code: NSJ-RQ7438
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7438-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Tropomyosin 2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 9
Immunofluorescent staining of FFPE human placental tissue with Tropomyosin 2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 9
IHC staining of FFPE human spleen tissue with Tropomyosin 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 9
IHC staining of FFPE human colorectal adenocarcinoma tissue with Tropomyosin 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 9
IHC staining of FFPE human placental tissue with Tropomyosin 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 9
IHC staining of FFPE human lung adenocarcinoma tissue with Tropomyosin 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 9
Western blot testing of 1) human HeLa, 2) rat H9C2(2-1) and 3) mouse skeletal muscle tissue lysate with Tropomyosin 2 antibody. Expected molecular weight: 33-37 kDa.
7 / 9
Flow cytometry testing of human SiHa cells with Tropomyosin 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Tropomyosin 2 antibody.
8 / 9
Flow cytometry testing of mouse ANA-1 cells with Tropomyosin 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Tropomyosin 2 antibody.
9 / 9
Flow cytometry testing of rat RH35 cells with Tropomyosin 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Tropomyosin 2 antibody.
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Immunofluorescent staining of FFPE human placental tissue with Tropomyosin 2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human spleen tissue with Tropomyosin 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colorectal adenocarcinoma tissue with Tropomyosin 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human placental tissue with Tropomyosin 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung adenocarcinoma tissue with Tropomyosin 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HeLa, 2) rat H9C2(2-1) and 3) mouse skeletal muscle tissue lysate with Tropomyosin 2 antibody. Expected molecular weight: 33-37 kDa.
Flow cytometry testing of human SiHa cells with Tropomyosin 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Tropomyosin 2 antibody.
Flow cytometry testing of mouse ANA-1 cells with Tropomyosin 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Tropomyosin 2 antibody.
Flow cytometry testing of rat RH35 cells with Tropomyosin 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Tropomyosin 2 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 2-5ug/ml
Application Note:
Optimal dilution of the Tropomyosin 2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Beta-Tropomyosin, also known as tropomyosin beta chain is a protein that in humans is encoded by the TPM2 gene. This gene encodes beta-tropomyosin, a member of the actin filament binding protein family, and mainly expressed in slow, type 1 muscle fibers. Mutations in this gene can alter the expression of other sarcomeric tropomyosin proteins, and cause cap disease, nemaline myopathy and distal arthrogryposis syndromes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids KQAEDRCKQLEEEQQALQK from the human protein were used as the immunogen for the Tropomyosin 2 antibody.
Limitation:
This Tropomyosin 2 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
P07951