SEC13 Antibody / SEC13L1 / SEC13-like protein 1

Contact us
NSJ Bioreagents
Product Code: NSJ-RQ7444
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7444-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the SEC13L1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 4
Immunofluorescent staining of FFPE human A549 cells with SEC13L1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
Western blot testing of 1) human HepG2, 2) human K562, 3) human HeLa and 4) mouse ovary tissue lysate with SEC13L1 antibody. Predicted molecular weight: 34-41 kDa (multiple isoforms).
3 / 4
Flow cytometry testing of human HL60 cells with SEC13L1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SEC13L1 antibody.
4 / 4
Flow cytometry testing of human JK cells with SEC13L1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SEC13L1 antibody.
❮❯

Immunofluorescent staining of FFPE human A549 cells with SEC13L1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HepG2, 2) human K562, 3) human HeLa and 4) mouse ovary tissue lysate with SEC13L1 antibody. Predicted molecular weight: 34-41 kDa (multiple isoforms).
Flow cytometry testing of human HL60 cells with SEC13L1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SEC13L1 antibody.
Flow cytometry testing of human JK cells with SEC13L1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SEC13L1 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the SEC13L1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Protein SEC13 homolog, also called SEC13-like protein 1 (SEC13L1) and SEC13-related protein (SEC13R) is a protein that in humans is encoded by the SEC13 gene. The protein encoded by this gene belongs to the SEC13 family of WD-repeat proteins. It is a constituent of the endoplasmic reticulum and the nuclear pore complex. It has similarity to the yeast SEC13 protein, which is required for vesicle biogenesis from endoplasmic reticulum during the transport of proteins. Multiple alternatively spliced transcript variants have been found.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids Q20-K305) was used as the immunogen for the SEC13L1 antibody.
Limitation:
This SEC13L1 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
P55735