VAPA Antibody / VAP33

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NSJ Bioreagents
Product Code: NSJ-RQ7451
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7451-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the VAPA antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 11
IHC staining of FFPE human cervical cancer tissue with VAPA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 11
IHC staining of FFPE human lung adenocarcinoma tissue with VAPA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 11
IHC staining of FFPE human colorectal adenocarcinoma tissue with VAPA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 11
IHC staining of FFPE human placenta tissue with VAPA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 11
IHC staining of FFPE mouse colon tissue with VAPA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 11
IHC staining of FFPE rat colon tissue with VAPA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 11
Immunofluorescent staining of FFPE human colorectal adenocarcinoma tissue with VAPA antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
8 / 11
Immunofluorescent staining of FFPE rat colon tissue with VAPA antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
9 / 11
Immunofluorescent staining of FFPE human PC-3 cells with VAPA antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
10 / 11
Western blot testing of 1) human HeLa, 2) human K562, 3) human 293T, 4) human HepG2, 5) rat brain and 6) mouse brain tissue lysate with VAPA antibody. Expected molecular weight: 28-33 kDa (two isoforms).
11 / 11
Flow cytometry testing of human U-87 MG cells with VAPA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= VAPA antibody.
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IHC staining of FFPE human cervical cancer tissue with VAPA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung adenocarcinoma tissue with VAPA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colorectal adenocarcinoma tissue with VAPA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human placenta tissue with VAPA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse colon tissue with VAPA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat colon tissue with VAPA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human colorectal adenocarcinoma tissue with VAPA antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE rat colon tissue with VAPA antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human PC-3 cells with VAPA antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human K562, 3) human 293T, 4) human HepG2, 5) rat brain and 6) mouse brain tissue lysate with VAPA antibody. Expected molecular weight: 28-33 kDa (two isoforms).
Flow cytometry testing of human U-87 MG cells with VAPA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= VAPA antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the VAPA antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
VAMP-Associated Protein A (Vesicle-Associated Membrane Protein-Associated Protein A), also called 33 kDa VAMP-associated protein (VAP33) is a protein that in humans is encoded by the VAPA gene. The protein encoded by this gene is a type IV membrane protein. It is present in the plasma membrane and intracellular vesicles. It may also be associated with the cytoskeleton. This protein may function in vesicle trafficking, membrane fusion, protein complex assembly and cell motility. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids KHEQILVLDPPTDLKFK from the human protein were used as the immunogen for the VAPA antibody.
Limitation:
This VAPA antibody is available for research use only.
Localization:
Cell surface, cytoplasmic, nuclear membrane
Purity:
Antigen affinity purified
Uniprot #:
Q9P0L0