USP16 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ7453
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7453-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the Ubiquitin carboxyl-terminal hydrolase 16 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human HeLa cells with Ubiquitin carboxyl-terminal hydrolase 16 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Western blot testing of human 1) HeLa, 2) HEL and 3) K562 cell lysate with Ubiquitin carboxyl-terminal hydrolase 16 antibody. Predicted molecular weight ~94 kDa.
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Flow cytometry testing of human HepG2 cells with Ubiquitin carboxyl-terminal hydrolase 16 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ubiquitin carboxyl-terminal hydrolase 16 antibody.
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Immunofluorescent staining of FFPE human HeLa cells with Ubiquitin carboxyl-terminal hydrolase 16 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HeLa, 2) HEL and 3) K562 cell lysate with Ubiquitin carboxyl-terminal hydrolase 16 antibody. Predicted molecular weight ~94 kDa.
Flow cytometry testing of human HepG2 cells with Ubiquitin carboxyl-terminal hydrolase 16 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ubiquitin carboxyl-terminal hydrolase 16 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Ubiquitin carboxyl-terminal hydrolase 16 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Ubiquitin carboxyl-terminal hydrolase 16 is an enzyme that in humans is encoded by the USP16 gene. It is mapped to 21q21.3. This gene encodes a deubiquitinating enzyme that is phosphorylated at the onset of mitosis and then dephosphorylated at the metaphase/anaphase transition. It can deubiquitinate H2A, one of two major ubiquitinated proteins of chromatin, in vitro and a mutant form of the protein was shown to block cell division. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids H103-Q683) was used as the immunogen for the Ubiquitin carboxyl-terminal hydrolase 16 antibody.
Limitation:
This Ubiquitin carboxyl-terminal hydrolase 16 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q9Y5T5