TPP2 Antibody / Tripeptidyl-peptidase 2

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NSJ Bioreagents
Product Code: NSJ-RQ7457
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7457-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Tripeptidyl-peptidase 2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Western blot testing of 1) rat liver, 2) rat testis, 3) rat brain, 4) rat RH35, 5) mouse liver, 6) mouse testis, 7) mouse brain and 8) mouse NIH 3T3 cell lysate with Tripeptidyl-peptidase 2 antibody. Predicted molecular weight ~138 kDa.
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Western blot testing of human 1) Jurkat, 2) HeLa, 3) HepG2, 4) HEL and 5) K562 cell lysate with Tripeptidyl-peptidase 2 antibody. Predicted molecular weight ~138 kDa.
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IHC staining of FFPE human colorectal adenocarcinoma tissue with Tripeptidyl-peptidase 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 7
IHC staining of FFPE human lung cancer tissue with Tripeptidyl-peptidase 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 7
IHC staining of FFPE human liver cancer tissue with Tripeptidyl-peptidase 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Immunofluorescent staining of FFPE human A549 cells with Tripeptidyl-peptidase 2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
7 / 7
Flow cytometry testing of human SiHa cells with Tripeptidyl-peptidase 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Tripeptidyl-peptidase 2 antibody.
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Western blot testing of 1) rat liver, 2) rat testis, 3) rat brain, 4) rat RH35, 5) mouse liver, 6) mouse testis, 7) mouse brain and 8) mouse NIH 3T3 cell lysate with Tripeptidyl-peptidase 2 antibody. Predicted molecular weight ~138 kDa.
Western blot testing of human 1) Jurkat, 2) HeLa, 3) HepG2, 4) HEL and 5) K562 cell lysate with Tripeptidyl-peptidase 2 antibody. Predicted molecular weight ~138 kDa.
IHC staining of FFPE human colorectal adenocarcinoma tissue with Tripeptidyl-peptidase 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with Tripeptidyl-peptidase 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with Tripeptidyl-peptidase 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human A549 cells with Tripeptidyl-peptidase 2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human SiHa cells with Tripeptidyl-peptidase 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Tripeptidyl-peptidase 2 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Tripeptidyl-peptidase 2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Tripeptidyl-peptidase 2 is an enzyme that in humans is encoded by the TPP2 gene. This gene encodes a mammalian peptidase that, at neutral pH, removes tripeptides from the N terminus of longer peptides. The protein has a specialized function that is essential for some MHC class I antigen presentation. The protein is a high molecular mass serine exopeptidase; the amino acid sequence surrounding the serine residue at the active site is similar to the peptidases of the subtilisin class rather than the trypsin class.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids E32-K1001) was used as the immunogen for the Tripeptidyl-peptidase 2 antibody.
Limitation:
This Tripeptidyl-peptidase 2 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Uniprot #:
P29144