SEC24C Antibody / SEC24-related protein C

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NSJ Bioreagents
Product Code: NSJ-RQ7466
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7466-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the SEC24C antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 3
Immunofluorescent staining of FFPE human MCF7 cells with SEC24C antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human 1) HeLa, 2) 293T, 3) Jurkat and 4) T-47D cell lysate with SEC24C antibody. Predicted molecular weight ~118 kDa.
3 / 3
Flow cytometry testing of human K562 cells with SEC24C antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SEC24C antibody.
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Immunofluorescent staining of FFPE human MCF7 cells with SEC24C antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HeLa, 2) 293T, 3) Jurkat and 4) T-47D cell lysate with SEC24C antibody. Predicted molecular weight ~118 kDa.
Flow cytometry testing of human K562 cells with SEC24C antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SEC24C antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the SEC24C antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Protein transport protein Sec24C is a protein that in humans is encoded by the SEC24C gene. The protein encoded by this gene is a member of the SEC24 subfamily of the SEC23/SEC24 family, which is involved in vesicle trafficking. The encoded protein has similarity to yeast Sec24p component of COPII. COPII is the coat protein complex responsible for vesicle budding from the ER. The product of this gene may play a role in shaping the vesicle, as well as in cargo selection and concentration. Alternatively spliced transcript variants encoding the same protein have been identified.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids L178-R1033) was used as the immunogen for the SEC24C antibody.
Limitation:
This SEC24C antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
P53992