THAP11 Antibody / THAP domain-containing protein 11

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NSJ Bioreagents
Product Code: NSJ-RQ7472
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7472-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the THAP11 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Western blot testing of 1) rat brain, 2) rat lung, 3) rat stomach, 4) rat L6, 5) mouse brain, 6) mouse lung, 7) mouse stomach and 8) mouse NIH 3T3 cell lysate with THAP11 antibody. Predicted molecular weight ~34 kDa, commonly observed at 34-44 kDa.
2 / 8
Western blot testing of human 1) Jurkat, 2) HeLa, 3) HepG2, 4) Daudi, 5) 293T, 6) Caco-2, 7) MCF7 and 8) SiHa cell lysate with THAP11 antibody. Predicted molecular weight ~34 kDa, commonly observed at 34-44 kDa.
3 / 8
IHC staining of FFPE human breast cancer tissue with THAP11 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 8
IHC staining of FFPE human colorectal adenocarcinoma tissue with THAP11 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 8
IHC staining of FFPE mouse brain tissue with THAP11 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 8
IHC staining of FFPE rat brain tissue with THAP11 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 8
Immunofluorescent staining of FFPE human A549 cells with THAP11 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
8 / 8
Flow cytometry testing of human MCF7 cells with THAP11 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= THAP11 antibody.
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Western blot testing of 1) rat brain, 2) rat lung, 3) rat stomach, 4) rat L6, 5) mouse brain, 6) mouse lung, 7) mouse stomach and 8) mouse NIH 3T3 cell lysate with THAP11 antibody. Predicted molecular weight ~34 kDa, commonly observed at 34-44 kDa.
Western blot testing of human 1) Jurkat, 2) HeLa, 3) HepG2, 4) Daudi, 5) 293T, 6) Caco-2, 7) MCF7 and 8) SiHa cell lysate with THAP11 antibody. Predicted molecular weight ~34 kDa, commonly observed at 34-44 kDa.
IHC staining of FFPE human breast cancer tissue with THAP11 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colorectal adenocarcinoma tissue with THAP11 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with THAP11 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with THAP11 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human A549 cells with THAP11 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human MCF7 cells with THAP11 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= THAP11 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the THAP11 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
The protein encoded by this gene contains a THAP domain, which is a conserved DNA-binding domain that has striking similarity to the site-specific DNA-binding domain (DBD) of Drosophila P element transposases.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids M1-E294) was used as the immunogen for the THAP11 antibody.
Limitation:
This THAP11 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q96EK4