CVAK104 Antibody / SCYL2

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NSJ Bioreagents
Product Code: NSJ-RQ7473
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7473-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the CVAK104 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 3
Immunofluorescent staining of FFPE human A549 cells with CVAK104 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human 1) HeLa, 2) MCF7, 3) 293T and 4) Jurkat cell lysate with CVAK104 antibody. Predicted molecular weight ~104 kDa.
3 / 3
Flow cytometry testing of human U-87 MG cells with CVAK104 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CVAK104 antibody.
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Immunofluorescent staining of FFPE human A549 cells with CVAK104 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HeLa, 2) MCF7, 3) 293T and 4) Jurkat cell lysate with CVAK104 antibody. Predicted molecular weight ~104 kDa.
Flow cytometry testing of human U-87 MG cells with CVAK104 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CVAK104 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the CVAK104 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
SCY1-like protein 2, also called Coated vesicle-associated kinase of 104 kDa and CVAK104, is a protein that in humans is encoded by the SCYL2 gene. The protein encoded by this gene associates with clathrin-coated complexes at the plasma membrane and with endocytic coated vesicles. The encoded protein phosphorylates the beta2 subunit of the plasma membrane adapter complex AP2 and interacts with clathrin, showing involvement in clathrin-dependent pathways between the trans-Golgi network and the endosomal system. In addition, this protein has a role in the Wnt signaling pathway by targeting frizzled 5 (Fzd5) for lysosomal degradation. Two transcript variants encoding the same protein have been found for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids E208-Q909) was used as the immunogen for the CVAK104 antibody.
Limitation:
This CVAK104 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q6P3W7