SEPT6 Antibody / Septin 6

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NSJ Bioreagents
Product Code: NSJ-RQ7480
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7480-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Septin 6 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 5
IHC staining of FFPE human tonsil tissue with Septin 6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human spleen tissue with Septin 6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
Western blot testing of 1) human SH-SY5Y, 2) human MOLT4, 3) rat thymus, 4) rat brain and 5) mouse brain tissue lysate with Septin 6 antibody. Predicted molecular weight ~50 kDa.
4 / 5
Flow cytometry testing of human HEL cells with Septin 6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Septin 6 antibody.
5 / 5
Flow cytometry testing of mouse ANA-1 cells with Septin 6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Septin 6 antibody.
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IHC staining of FFPE human tonsil tissue with Septin 6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human spleen tissue with Septin 6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human SH-SY5Y, 2) human MOLT4, 3) rat thymus, 4) rat brain and 5) mouse brain tissue lysate with Septin 6 antibody. Predicted molecular weight ~50 kDa.
Flow cytometry testing of human HEL cells with Septin 6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Septin 6 antibody.
Flow cytometry testing of mouse ANA-1 cells with Septin 6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Septin 6 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Septin 6 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Septin-6 is a protein that in humans is encoded by the SEPT6 gene. This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis. One version of pediatric acute myeloid leukemia is the result of a reciprocal translocation between chromosomes 11 and X, with the breakpoint associated with the genes encoding the mixed-lineage leukemia and septin 2 proteins. This gene encodes four transcript variants encoding three distinct isoforms. An additional transcript variant has been identified, but its biological validity has not been determined.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids M1-E434) was used as the immunogen for the Septin 6 antibody.
Limitation:
This Septin 6 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
Q14141