THBS3 Antibody / Thrombospondin-3

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NSJ Bioreagents
Product Code: NSJ-RQ7484
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7484-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Thrombospondin-3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human breast cancer tissue with Thrombospondin-3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human liver cancer tissue with Thrombospondin-3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of human 1) SH-SY5Y and 2) MCF7 cell lysate with Thrombospondin-3 antibody. Predicted molecular weight ~104 kDa but may be observed at higher molecular weights due to glycosylation.
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Flow cytometry testing of human MCF7 cells with Thrombospondin-3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Thrombospondin-3 antibody.
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IHC staining of FFPE human breast cancer tissue with Thrombospondin-3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with Thrombospondin-3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) SH-SY5Y and 2) MCF7 cell lysate with Thrombospondin-3 antibody. Predicted molecular weight ~104 kDa but may be observed at higher molecular weights due to glycosylation.
Flow cytometry testing of human MCF7 cells with Thrombospondin-3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Thrombospondin-3 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Thrombospondin-3 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Thrombospondin-3 (TSP3) is a protein that in humans is encoded by the THBS3 gene. The protein encoded by this gene belongs to the thrombospondin family. Thrombospondin family members are adhesive glycoproteins that mediate cell-to-cell and cell-to-matrix interactions. This protein forms a pentameric molecule linked by a single disulfide bond. This gene shares a common promoter with metaxin 1. Alternate splicing results in coding and non-coding transcript variants.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids Q23-T486) was used as the immunogen for the Thrombospondin-3 antibody.
Limitation:
This Thrombospondin-3 antibody is available for research use only.
Localization:
Perinuclear, extracellular
Purity:
Antigen affinity purified
Uniprot #:
P49746